This study aimed to evaluate the effect of Artemisia herba alba extracts (aqueous, alcoholic and essential oil) on

antibiotic-resistant bacteria. In which four types were tested, namely Staphylococcus aureus (MRSA)

Staphylococcus epidermidis, Klebsiella pneumoniae, Klebsiella oxytoca, using the diffusion disk method and

concentrations (100, 200, 300 mg/ml) for the aqueous and alcoholic extract, while the essential oil was used in

its pure form. The most effective antibacterial activity was observed for the essential oil of plant with an inhibition

diameter from 8.00±0.00 to 11.33±1.52 mm, and its aqueous extract effect on bacteria MRSA and S. epidermidis

was 8.66±1.52 and 8.33±0.57 mm, respectively, at 300 mg/ml. The inhibitory effect of alcoholic extract was

10.00±0.00 mm at 300 mg/ml for MRSA as for S. epidermidis at the same concentration had high inhibition

diameter 13.66±1.15 mm. Phytochemical screention of aqueous extract revealed the presence of phenols and

saponins, whereas, alcoholic extract revealed the presence of phenols, tannins, terpenoids and saponins. The

chemical components of the essential oils were determined by GC-MS device. The main compounds of A. herba

alba were Camphor (30.527%), Thujone (22.471%) and Camphene (10.291%). These results suggest that

essential oil extract can be considered as a potential source of antibacterial compounds.

Artemisia herba-alba Asso is a medicinal plant renowned for its therapeutic essential oil. This study characterized the chemical profile and bioactivities of the essential oil from Libyan A. herba-alba. The essential oil, obtained via hydrodistillation with a 2% yield, was analyzed by GC-MS/FID and evaluated for antibacterial (disc diffusion) and anticancer (MTT assay) properties. The oil was dominated by camphor (30.53%) and Thujone (22.47%), classifying it as a camphor-thujone chemotype. It exhibited remarkable antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA; 17.33 ± 1.52 mm), and demonstrated potent, dose-dependent cytotoxicity against MCF-7 breast cancer cells with an IC₅₀ of 0.59 mg/mL. The results validate the traditional use of this plant and highlight its potential as a source of antimicrobial and anticancer agents.

This research endeavor delved deeply into the potential protective efficacy of moringa extract in ameliorating renal impairments instigated by a high-fat dietary regimen in male rat models. The subjects were judiciously classified into six discrete cohorts, each comprising six individuals, with the subsequent allocation: Group 1 serving as the non-intervention control; Group 2 receiving a daily dose of 300 mg/kg body weight moringa extract (ME) spanning 8 weeks; Group 3 subjected to a sustained high-fat diet (HFD) throughout an 8-week interval; Group 4 exposed to a dual-modality involving an HFD and daily administration of 300 mg/kg bw ME for the identical duration; Group 5 subjected to the combined impact of an HFD and a daily 40 mg/kg bw dose of simvastatin (SIM) across 8 weeks; lastly, Group 6 subjected to a concurrent treatment approach involving an HFD, 300 mg/kg bw ME, and 40 mg/kg bw SIM, daily, over an 8-week period. Through an intricately orchestrated sequence of experiments, we embarked upon an expedition to unearth the Reno protective potential of moringa extract against dietary-induced nephrological impairment. Our findings offer an all-encompassing outlook on the synergy between dietary interventions and the innovative agents under scrutiny. This inquiry not only advances our comprehension of potential remedies for diet-associated renal adversities but also accentuates the emergence of moringa extract as a formidable contender in this domain. Our revelations illuminated that the application of a high-fat diet ushered in a substantial surge in malondialdehyde (MDA) levels, emblematic of heightened oxidative stress. This was concomitant with a marked depletion in glutathione (GSH), aggregate antioxidant capacity (TAC), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione reductase (GSH-Rd) levels. Conversely, the administration of moringa extract adeptly mitigated these adverse repercussions induced by the high-fat dietary regimen.

In aquaculture, various natural feed additives are used to achieve farmed fish sustainability and maintaining a good health status, stimulate immunity, and prevent diseases of the cultured fish with better growth and less cost. Plant essential oils attract attention among these natural products as feed additives, as they are considered to be safe for animals, humans, and the environment. Besides that, many plant oils have used as a source partially or completely replace fish oil for reducing the cost of fish meals, yielding improved fish growth, fish health and survival after bacterial infection. The present article gives an idea about the recent studies that utilize plant essential oils as feed additives in many cultured fish species with different initial body weight, dose and duration.

This review will highlight the chemical constituents and detail description of morphology and the pharmacological and therapeutic effects of Myrtus communis. This plant commonly known as myrtle, is an evergreen and aromatic herb of the family Myrtaceae, it is widely distributed throughout the world. M. communis was reported to contain carbohydrates (88.69%), crude protein (5.97%), crude oil (3.59%), moisture content (63.67%) and total ash (1.75%) and mineral elements and many other bioactive contents. Manypharmacological activities viz., antibacterial, antifungal, anticancer, antiviral, antioxidant, antidiabetic, hepatoprotective and neuroprotective etc. have been reported in different plant extracts.

Medicinal herbs are used for growth promotion, disease control and other health benefits in aquaculture industry.

Here, we examined the effect of dietary laurel-leaf cistus (Cistus laurifolius) ethanolic extract on growth

performance, digestive enzyme activity, haematological profile and nonspecific immune responses in common

carp (Cyprinus carpio). In addition, resistance against Aeromonas hydrophila infection was examined. Common

carp was fed diets containing 0 (Control), 0.1 (CL0.1), 0.5 (CL0.5) and 1 (CL1) g kg􀀀 1 laurel-leaf cistus extract for

45 days. After 30 days, superoxide anion production (SAP) increased in CL0.1 and CL0.5 fish groups and at the

end of the study all experimental fish groups had higher SAP compared to that of the control (P ˂ 0.05).

Lysozyme activity (LA) was elevated in CL0.5 and CL1 treated groups on 30th day (P < 0.05), and this increase

was only observed in C0.1 fish group at the end of study compared to control (P ˂ 0.05). Myeloperoxidase activity

was significantly increased in CL0.5 and CL1 fish groups at the end of study. IL-1βgene expression was

significantly increased in treated fish in a dose-depended manner. Similar results were observed for transcription

of IL-6 and IL-8 (P < 0.05). Anti-inflammatory cytokines, IL-10 and TGF-β were highly up-regulated in the intestine

and head kidney of CL treated fish groups compared to control (P < 0.05). At the end of experiment,

significantly higher final body weight, weight gain, and specific growth rate were obtained in CL0.1 treated fish

group compared to control. However, growth was negatively affected in CL1 fish group (P < 0.05). CL1 fish

group had also a significantly higher FCR. Amylase activity was significantly increased in all experimental fish

groups compared to control (P ˂ 0.05). Trypsin activity was decreased in CL0.1 and CL1 fish groups (P ˂ 0.05).

WBC and RBC were significantly increased (P ˂ 0.05) in CL0.5 and CL1 fish groups, whereas haemoglobin,

haematocrit, mean cell, mean cell haemoglobin contents were no significantly changed among control and

treatment groups. Result of challenge test with A. hydrophila exhibited that survival rate in all treatment groups

was significantly higher than that of control. These findings demonstrated that laurel-leaf cistus at 0.1 g kg􀀀 1 can

be a suitable candidate for growth promotion, immune system induction and infection control in fish.

Abstract: This study was designed to evaluate the growth and digestive enzyme activity parameters of rainbow trout juvenile Oncorhynchus mykiss fed diets containing different levels (0%, 0.1%, 0.5% or 1%) of Glycyrrhiza glabra, Coriandrum sativum and Cassia angustifolia aqueous methanolic extract as a feed additive seventy-five days. The fish with initial weight of 22.65 ± 0.07 g were divided into 30 tanks so that 10 groups would be formed and stored as 50 fish in each tank, so the experiment was started as three replications. At the end of every month, samples are collected from the digestive system for use in measuring digestive enzymes, and scales and weights for use in measuring growth rate. Digestive enzymes like pepsin, trypsin, amylase and lipase and growth parameters such as final weight, weight gain (WG), specific growth rate (SGR), feed conversion ratio (FCR). Amylase, lipase, pepsin and trypsin are different in terms of enzyme activities. The effect of medicinal plants gave a good indication of improvement in the effectiveness of digestive enzymes with the length of the experiment during the surrounding environment. The values related to the growth parameters were lower than the control group in the study where similar or low values were encountered.

In this study, the effect of a dietary supplementation of Apium graveolens on growth performance, digestive enzyme activities and immune response of juvenile common carp (Cyprinus carpio) were evaluated. C. carpio, with an average weight at the beginning of the experiment of 6.5 ± 0.07 g, were supplemented with a celery (Apium graveolens) aqueous methanolic extract at a dose of 0% (control), 0.1%, 0.5% or 1% over 45 days. Every 15 days, respiratory burst, lysozyme and myeloperoxidase activity were measured, and at the end of the study, haematological responses, growth performance and digestive enzyme activity were investigated. Respiratory burst and myeloperoxidase activity was significantly improved in all treated groups compared with the control group (P ˂ 0.05). Lysozyme activity was highest in the 0.1% and 1% extract groups. At the end of the study, the final weight (FW), weight gain (WG) and specific growth rate (SGR) were significantly increased in the 0.1% group compared with the other groups. The feed concentration ratio was decreased in the 0.1% and 1% groups compared with the control (P < 0.05). Haematological parameters were affected by A. graveolens extract intake (P ˂ 0.05). The total white and red blood counts, haemoglobin content and haematocrit value were highest in the 0.1% extract group (P ˂ 0.05). However, the mean cell volume and mean cell haemoglobin concentration were decreased in the 0.5% group (P ˂ 0.05). Digestive enzyme activity (trypsin, amylase and lipase) in all experimental groups was significantly elevated compared with the control (P ˂ 0.05). These results indicated an immunostimulatory and growth-promoting effect and increasing digestive enzyme activity of C. carpio supplemented with an A. graveolens aqueous methanolic extract.

This study was conducted to determine the effect of dietary supplementation with Tilia tomentosa on the growth performance, digestive enzyme activity, haematological indices and nonspecific immune indices of juvenile common carp (Cyprinus carpio). Fish with an average weight of 4.35 ± 0.16 g were fed a diet supplemented with an aqueous methanolic extract of T. tomentosa at a dose of 0% (control), 0.01%, 0.05% or 0.1% for 45 days. The final weight, weight gain and specific growth rate were observed to be significantly higher for the 00.1% and 0.1% groups compared with the control group (P < 0.05). The feed conversion ratio was significantly decreased in the 0.05% and 0.1% groups compared with the control (P < 0.05). The activities of various digestive enzymes (amylase, lipase and trypsin) were also measured and no significant differences were observed compared to the control (P > 0.05). The mean cell volume of the 0.01% group was significantly increased compared to the control (P < 0.05) and increased lysozyme activity was observed in the 0.05% and 0.1% groups. Respiratory burst activity was significantly increased (P < 0.05) on days 15 and 30 for the 0.1% and 0.05% groups, respectively. No differences were observed for myeloperoxidase activity among the four groups. These results suggest that aqueous methanolic extract of T. tomentosa has a growth-promoting and immunostimulatory effect on common carp.

The present study was conducted to investigate the efficacy of Chenopodium album aqueous methanolic extract supplementation on the immunological and haematological indices, digestive enzyme activity and growth performance of the common carp (Cyprinus carpio). C. album was added to a basal diet at the rate of 0 (CA0), 0.01 (CA0.01), 0.05 (CA0.05) and 0.1 g kg−1 (CA0.1), and C. carpio was fed this diet for 45 days. Respiratory burst activity was significantly increased in all experimental groups on days 15 and 30 compared to the control (P < 0.05). Lysozyme activity was significantly increased over all sampling times compared to the control except in CA0.1 (P < 0.05). Myeloperoxidase activities were significantly increased in all experimental groups compared to

Abstract: This study was conducted to determine the effect of a dietary supplementation of Tilia tomentosa on blood parameters and non-specific immune responses in common carp (Cyprinus carpio). With this aim, common carp (4.35 ± 0.16 g) were fed with an average weight at the beginning of the experiment of 4.35 ± 0.16 g, were supplemented with a celery (Tilia tomentosa) aqueous methanolic extract at a dose of 0% (control), 0.01%, 0.05% or 0.1% during 45 days. Every 15 days of the study, blood indices (WBC, RBC, Hb, Hct, MCV, MCH, and MCHC), and immune parameters such as lysozyme, respiratory burst, and myeloperoxidase activities were investigated. The results indicated that fish fed diet supplemented with 0.01%, 0.05%, and 0.1% T. tomentosa extract had no significant differences compared with the control on the 15th, 30th, and 45th days of the experiment (P > 0.05) except MCV of 0.1% on the 30th day of the experiment which had highest recorded value compared with other groups (P ˂ 0.05). MCHC of the control on the 30th day of the experiment also had highest recorded result compared with other groups. (P ˂ 0.05). Lysozyme activity was highest in the 0.01% and 0.1% experimental groups (P ˂ 0.05). At the end of the study, respiratory burst activity decreased on the 15th day and 30th day of the study on 0.01% and 0.1% groups (P ˂ 0.05). Myeloperoxidase activity was not affected in all experimental groups compared to control (P > 0.05). These results suggested that Tilia tomentosa has some immunomodulatory effects in common carp

Abstract: The present study was conducted to investigate the efficacy of Chenopodium album methanolic extract supplemented with fish diet on non-specific immune activity and hematological parameters of Common carp (Cyprinus carpio) juveniles. Common carp (2.4 ± 0.1 g) were divided into four main groups in triplicate (twelve aquariums, 40 fish per aquarium) fed diet containing three doses of C. album extract, CA 0, CA 0.01%, CA 0.05% and CA 0.1% kg diet individually for 45 days. The samples were collected every 15 days. The results indicated that Nitroblue tetrazolium (NBT) activity was significantly increased in all experimental groups at 15 and 30 days, lysozyme activity was significantly increased in all the experimental groups at 15 days. While, Myeloperoxidase (MPO) activity was significantly improved (P < 0.05) in all experimental groups at all experimental periods, 15, 30 and 45 days compared to the control groups. Regarding hematological parameters, a significantly decreased in WBC and insignificantly changes in (RBC, Hb, HCT, MCH, MCV and MCHC in all experimental groups compared with the control. The results indicated that the supplementation of methanolic extract of Chenopodium album to carp diet was beneficial because it increases innate immune response.

Abstract: In this study, the effect of a dietary supplementation of Apium graveolens on digestive enzyme activities and growth performance of juvenile common carp (Cyprinus carpio) were evaluated. C. carpio, with an average weight at the beginning of the experiment of 6.5 ± 0.07 g, were supplemented with a celery (Apium graveolens) aqueous methanolic extract at a dose of 0% (control), 0.01%, 0.05% or 0.1% over 45 d. At the end of the study, digestive enzyme activity and growth performance were investigated. Results of this study showed that the final weight, weight gain and specific growth rate were significantly increased (P < 0.05) in the 0.01% group compared with the other groups. The feed concentration ratio was decreased in the 0.01% and 1% groups compared with the control (P < 0.05). Digestive enzyme activity (trypsin, amylase and lipase) in all experimental groups was significantly elevated compared with the control (P ˂ 0.05). These results indicated growth-promoting effect and increasing digestive enzyme activity of C. carpio supplemented with an A. graveolens aqueous methanolic extract. 

Abstract: The digestive enzymes activity and the growth performance effect to the methanolic extract of Chenopodium album supplementation were examined in common carp (Cyprinus carpio) juveniles. Common carp (2.4 ± 0.1 g) were fed diets containing three doses of Chenopodium album extract (CA 0, CA 0.01%, CA 0.05% and CA 0.1% / kg of feed) individually for 45 days. The results indicated that trypsin and lipase activities were a significantly increased in 0.5% and 1% treated groups. While, amylase activity was significantly improved in all treated groups compared to the control. Final weight and weight gain were significantly enhanced in 0.01% and 0.1% treated groups. FCR and SR were insignificant changes in all experimental groups. While, SGR was significantly increased in all treated groups compared to the control. The results concluded that the supplementation of methanolic extract of Chenopodium album to carp diet could stimulate the digestive enzymes secretion and some growth performance parameters.

Abstract: In this study, the effect of a dietary supplementation of Apium graveolens on immune and haematological responses of juvenile common carp (Cyprinus carpio) were evaluated. C. carpio, with an average weight at the beginning of the experiment of 6.5 ± 0.07 g, were supplemented with a celery (Apium graveolens) aqueous methanolic extract at a dose of 0% (control), 0.01%, 0.05% or 0.1% over 45 d. Every 15 d, respiratory burst, lysozyme and myeloperoxidase activity were measured, and at the end of the study, haematological responses were investigated. The result showed that respiratory burst and myeloperoxidase activity was significantly improved in all treated groups compared with the control group (P ˂ 0.05). Lysozyme activity was highest in the 0.01% and 0.1% extract groups. Haematological parameters were affected by A. graveolens extract intake (P ˂ 0.05). The total white and red blood counts, haemoglobin content and haematocrit value were highest in the 0.1% extract group (P ˂ 0.05). However, the mean cell volume, mean cell haemoglobin and mean cell haemoglobin concentration were decreased in the 0.05% group (P ˂ 0.05). These results indicated an immunostimulatory effect of A. graveolens aqueous methanolic extract for common carp. 

Abstract: In this study, the effect of a dietary supplementation of Tilia tomentosa on growth performance and digestive enzyme activities in juvenile common carp (Cyprinus carpio) were evaluated. C. carpio, with an average weight of 4.35 ± 0.16 g, were fed diet with supplemented with a celery (Tilia tomentosa) aqueous methanolic extract at the dose of 0 mg kg-1 (control), 100 mg kg-1 , 500 mg kg-1 and 1000 mg kg-1 during 45 days. At the end of the study, the final weight was significantly increased in the 100 mg kg-1 , and 1 mg kg-1 compared with the control group (P < 0.05). The weight gain rate was also significantly increased in the experimental groups’ compared with the control (P < 0.05). The feed conversion ratio was significantly decreased in the 500 mg kg-1 and 1000 mg kg-1 groups compared with the control (P < 0.05). The specific growth rate was significantly increased in the 500 mg kg-1 and 1000 mg kg-1 groups compared with control group (P < 0.05). The digestive enzymes activity (Amylase, lipase and trypsin) was also measured and no significant differences in the enzymes activities were determined at the end of the study compared to control (P > 0.05). 

Abstract: The present study was carried out to examine the effect of aqueous suspension of

Cichorium endivia leaves powder or glibenclamide drug on some biochemical parameters in

streptozotocin-induced diabetic rats. Adult male albino rats were randomly divided into 5 groups, (1)

control group which was administered distilled water, (2) Cichorium endivia group in which rats

received aqueous suspension of C. endivia leaves powder, 500 mg/kg b. wt., (3) diabetic group in

which diabetic rats were administered distilled water, (4) diabetic–Cichorium endivia group in which

diabetic rats were treated with aqueous suspension of C. endivia leaves powder, 500 mg/kg b. wt.,

and (5) diabetic–glibenclamide group in which diabetic rats were treated with glibenclamide drug, 600

mg/kg b. wt. All groups were induced by intragastric administration for six weeks. The C. endivia

leaves powder or the glibenclamide drug produced significant hepatoprotective effects by decreasing

the activities of serum aminotransferases (AST and ALT), alkaline phosphatase (ALP), lactate

dehydrogenase (LDH) and liver malondialdehyde (MDA) level as well as liver superoxide dismutase

(SOD) and catalase (CAT) activities, and increasing the liver glutathione peroxidase (GPx) and

glutathione-S-transferase (GST) activities and reduced glutathione (GSH) level. In conclusion, the

results obtained clearly indicate the role of oxidative stress in induction of diabetes and suggest

protective and/or ameliorative effects of C. endivia leaves powder aqueous suspension, similar to the

diabetic drug (glibenclamide) effects in this animal model.